1U24
Crystal structure of Selenomonas ruminantium phytase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL12B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL12B2 |
| Detector technology | CCD |
| Collection date | 2002-10-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9797 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.469, 140.378, 80.680 |
| Unit cell angles | 90.00, 102.85, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.000 |
| Rwork | 0.180 |
| R-free | 0.22100 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.680 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.085 | |
| Number of reflections | 99831 | |
| <I/σ(I)> | 11.6 | |
| Completeness [%] | 93.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 0.1M sodium chloride, 0.1M sodium acetate trihydrate, 12% PEG 6000, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
