1U1Y
Crystal structure of a complex between WT bacteriophage MS2 coat protein and an F5 aptamer RNA stemloop with 2aminopurine substituted at the-10 position
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2000-05-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.244 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 288.610, 288.610, 654.680 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.850 |
| R-factor | 0.188 |
| Rwork | 0.188 |
| R-free | 0.19600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ms2 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.020 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Number of reflections | 170082 | |
| Completeness [%] | 60.8 | 71.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 303 | MS2 coat protein in 1.25% or 1.5% PEG 8K, sodium phosphate buffer., pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
