1U1V
Structure and function of phenazine-biosynthesis protein PhzF from Pseudomonas fluorescens 2-79
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 56.260, 56.260, 156.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.210 - 1.700 |
| R-factor | 0.1309 |
| Rwork | 0.130 |
| R-free | 0.15338 |
| Structure solution method | SAD |
| RMSD bond length | 0.025 |
| RMSD bond angle | 1.562 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 31965 | |
| <I/σ(I)> | 27.4 | 9.8 |
| Completeness [%] | 98.2 | 91.1 |
| Redundancy | 20.6 | 15.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 292 | 0.1M Na-Citrate, 0.2M Ammonium Sulphate, 10% (w/v) PEG 3350, pH 5.6, temperature 292K, VAPOR DIFFUSION, HANGING DROP |
