1U1B
Structure of bovine pancreatic Ribonuclease A in complex with 3'-phosphothymidine (3'-5')-pyrophosphate adenosine 3'-phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2003-10-09 |
| Detector | Nonius Kappa CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.086, 75.861, 51.821 |
| Unit cell angles | 90.00, 106.31, 90.00 |
Refinement procedure
| Resolution | 17.730 - 2.000 |
| R-factor | 0.218 |
| Rwork | 0.218 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qhc |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.600 | 2.130 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.092 | |
| Number of reflections | 14950 | |
| <I/σ(I)> | 16.1 | |
| Completeness [%] | 91.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 27% PEG 4000, 20 mM sodium citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
