1U1B
Structure of bovine pancreatic Ribonuclease A in complex with 3'-phosphothymidine (3'-5')-pyrophosphate adenosine 3'-phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | OTHER |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2003-10-09 |
Detector | Nonius Kappa CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 31.086, 75.861, 51.821 |
Unit cell angles | 90.00, 106.31, 90.00 |
Refinement procedure
Resolution | 17.730 - 2.000 |
R-factor | 0.218 |
Rwork | 0.218 |
R-free | 0.25800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qhc |
RMSD bond length | 0.008 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.600 | 2.130 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.092 | |
Number of reflections | 14950 | |
<I/σ(I)> | 16.1 | |
Completeness [%] | 91.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 27% PEG 4000, 20 mM sodium citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |