1U10
MEPA, active form with ZN in P1
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-27 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9791, 0.9793, 1.2810 |
| Spacegroup name | P 1 |
| Unit cell lengths | 35.613, 77.988, 127.663 |
| Unit cell angles | 93.15, 95.93, 90.75 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.23551 |
| Rwork | 0.234 |
| R-free | 0.26580 |
| Structure solution method | MAD combined with MR |
| Starting model (for MR) | MepA in the P21 form (1TZP) |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.615 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.039 | 0.151 |
| Number of reflections | 103423 | |
| <I/σ(I)> | 14.1 | 4 |
| Completeness [%] | 97.1 | 93.5 |
| Redundancy | 1.9 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | ammonium sulfate, MES, PEG monomethyl ether 5000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
