1U0J

Crystal Structure of AAV2 Rep40-ADP complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	109
Detector technology	CCD
Collection date	2003-05-15
Detector	BRANDEIS - B4
Wavelength(s)	1.1
Spacegroup name	P 65
Unit cell lengths	71.000, 71.000, 95.396
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.800 - 2.100
R-factor	0.192
Rwork	0.192
R-free	0.23700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1S9H (chain B)
RMSD bond length	0.018
RMSD bond angle	2.500
Data reduction software	HKL-2000
Data scaling software	NOVEL_R
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	50.000
High resolution limit [Å]	2.100	2.100
Rmerge	0.062	0.062
Number of reflections	15662
<I/σ(I)>	17.6	17.6
Completeness [%]	97.9	97.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	PEG 8k, sodium acetate, MgCl2, Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 4K