1U0J
Crystal Structure of AAV2 Rep40-ADP complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 109 |
| Detector technology | CCD |
| Collection date | 2003-05-15 |
| Detector | BRANDEIS - B4 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 65 |
| Unit cell lengths | 71.000, 71.000, 95.396 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.800 - 2.100 |
| R-factor | 0.192 |
| Rwork | 0.192 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1S9H (chain B) |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | NOVEL_R |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.062 | 0.062 |
| Number of reflections | 15662 | |
| <I/σ(I)> | 17.6 | 17.6 |
| Completeness [%] | 97.9 | 97.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | PEG 8k, sodium acetate, MgCl2, Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 4K |
