1U0F
Crystal structure of mouse phosphoglucose isomerase in complex with glucose 6-phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-08-14 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.400, 116.800, 73.900 |
| Unit cell angles | 90.00, 101.60, 90.00 |
Refinement procedure
| Resolution | 45.000 - 1.600 |
| R-factor | 0.18 |
| Rwork | 0.178 |
| R-free | 0.20700 |
| Structure solution method | REFINEMENT FROM THE NATIVE STRUCTURE |
| Starting model (for MR) | NATIVE STRUCTURE |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.213 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.057 | 0.369 |
| Number of reflections | 143578 | |
| <I/σ(I)> | 34.1 | 3.1 |
| Completeness [%] | 94.7 | 79.9 |
| Redundancy | 6 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 294 | 1.9 M ammonium sulphate, 100 mM Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
