1U0E
Crystal structure of mouse phosphoglucose isomerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-07-01 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.200, 116.100, 73.100 |
| Unit cell angles | 90.00, 101.30, 90.00 |
Refinement procedure
| Resolution | 14.960 - 1.600 |
| R-factor | 0.213 |
| Rwork | 0.211 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1n8t |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.160 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | d*TREK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.700 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.075 | 0.422 |
| Number of reflections | 147857 | |
| <I/σ(I)> | 5.4 | 1.5 |
| Completeness [%] | 99.5 | 96.4 |
| Redundancy | 5.4 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 294 | 1.9 M ammonium sulphate, 100 mM Tris-HCl, pH 8.5 , VAPOR DIFFUSION, HANGING DROP, temperature 294K |
