1U0B
Crystal structure of cysteinyl-tRNA synthetase binary complex with tRNACys
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-01-24 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 129.490, 129.490, 91.020 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.910 - 2.300 |
| R-factor | 0.25 |
| Rwork | 0.233 |
| R-free | 0.27700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1li5 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.860 | 2.410 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.088 | 0.504 |
| Number of reflections | 31401 | |
| <I/σ(I)> | 33.6 | 4.08 |
| Completeness [%] | 90.1 | 96.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 277 | Ammonium Sulfate, PEG 1000, Hepes, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
