1U09

Footand Mouth Disease Virus RNA-dependent RNA polymerase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID13
Synchrotron site	ESRF
Beamline	ID13
Temperature [K]	100
Detector technology	CCD
Collection date	2002-11-30
Detector	MARRESEARCH
Wavelength(s)	0.97967, 0.968623, 0.979851
Spacegroup name	P 41 21 2
Unit cell lengths	93.036, 93.036, 119.048
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.640 - 1.910
R-factor	0.21
Rwork	0.210
R-free	0.23500
Structure solution method	crystal 1: single wavelength protocol, crystal 2: MAD protocol, MAD with data collected at 0.97967, 0.968623, 0.979851 A
RMSD bond length	0.005
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.900
Rmerge	0.087
Number of reflections	40806
<I/σ(I)>	9.8
Completeness [%]	98.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	PEG 4000, Sodium Acetate, Sodium Citrate, butirolactone, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K