1U02
Crystal structure of trehalose-6-phosphate phosphatase related protein
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792, 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 117.995, 45.056, 53.391 |
| Unit cell angles | 90.00, 90.65, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.920 |
| Rwork | 0.197 |
| R-free | 0.21610 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.277 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (& sharp) |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.990 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.077 | 0.270 |
| Number of reflections | 21559 | |
| <I/σ(I)> | 14.7 | |
| Completeness [%] | 99.2 | 97.4 |
| Redundancy | 5.7 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 294 | PEG 4000, magnesium, chloride, Tris-HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
