1TZX
T. maritima NusB, P3221
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-04-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.05 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 60.011, 60.011, 172.442 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.720 |
| R-factor | 0.21862 |
| Rwork | 0.217 |
| R-free | 0.24937 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 1EYV |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.082 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.740 | 1.820 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Number of reflections | 39263 | |
| <I/σ(I)> | 37.6 | 2.9 |
| Completeness [%] | 99.6 | 99.5 |
| Redundancy | 4.7 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.9 | 277 | Sodium citrate, pH 7.9, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
