1TZT
T. maritima NusB, P21
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-04-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.05 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.714, 34.354, 74.322 |
| Unit cell angles | 90.00, 94.18, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.550 |
| R-factor | 0.23574 |
| Rwork | 0.235 |
| R-free | 0.25096 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 1EYV |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.181 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.650 |
| High resolution limit [Å] | 1.550 | 1.550 |
| <I/σ(I)> | 28.7 | 1.6 |
| Completeness [%] | 97.9 | 95.2 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.3 | 293 | ammonium sulfate, PEG 8000, pH 6.3, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
