1TZF

X-ray Crystal Structure of alpha-D-glucose-1-phosphate cytidylyltransferase from Salmonella typhi

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	130
Detector technology	CCD
Collection date	2004-03-29
Detector	SBC-3
Wavelength(s)	0.96410
Spacegroup name	P 63 2 2
Unit cell lengths	88.500, 88.500, 162.300
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.100
R-factor	0.199
Rwork	0.197
R-free	0.24800
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	2.200
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Number of reflections	22358
<I/σ(I)>	65.3	9.9
Completeness [%]	98.5	98.1
Redundancy	14.1	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	277	PEG 4000, Ammonium sulfate, sodium acetate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K