1TYZ

Crystal structure of 1-Aminocyclopropane-1-carboyxlate Deaminase from Pseudomonas

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	CCD
Collection date	2002-11-12
Detector	ADSC QUANTUM 4
Wavelength(s)	1.1000
Spacegroup name	P 21 21 21
Unit cell lengths	67.721, 68.651, 350.548
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.730 - 2.000
R-factor	0.215
Rwork	0.215
R-free	0.25100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1f2d
RMSD bond length	0.011
RMSD bond angle	1.600
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.070
High resolution limit [Å]	2.000	2.000
Number of reflections	98751
<I/σ(I)>	29.4	4.8
Completeness [%]	87.5	50
Redundancy	6.8	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	ISOPROPANOL, PEG 4000, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K