1TYU

STRUCTURE OF TAILSPIKE-PROTEIN

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1995-08
Detector	MARRESEARCH
Spacegroup name	P 21 3
Unit cell lengths	120.900, 120.900, 120.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.800
R-factor	0.184
Rwork	0.184
RMSD bond length	0.009
RMSD bond angle	1.600
Data reduction software	MOSFLM
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	1.800 *	1.800 *
Rmerge	0.061	0.293 *
Total number of observations	276996 *
Number of reflections	51290
Completeness [%]	93.5	36.5 *
Redundancy	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	10 *	4 *	drop contained 0.005 ml of drop solution and 0.003 ml of precipitant *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	HEPES	10 (mM)
3	1	drop	ammonium sulfate	1.5 (M)	precipitant
4	1	drop	sodium phosphate	0.1 (M)	precipitant
5	1	reservoir	ammonium sulfate	1.0 (M)
6	1	reservoir	sodium phosphate	0.1 (M)