1TXU

Crystal Structure of the Vps9 Domain of Rabex-5

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12B
Synchrotron site	NSLS
Beamline	X12B
Temperature [K]	100
Detector technology	CCD
Collection date	2003-10-23
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97956,0.97944,0.96805,0.97954,0.97948,0.99530
Spacegroup name	P 21 21 2
Unit cell lengths	68.047, 88.329, 47.299
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.350
R-factor	0.229
Rwork	0.227
R-free	0.26900
Structure solution method	MAD
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.420
High resolution limit [Å]	2.350	2.350
Number of reflections	11599
<I/σ(I)>	23.3	2
Completeness [%]	93.7	74.5
Redundancy	4	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	PEG 8000, MgCl, TRIS, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 4K