1TXQ
Crystal structure of the EB1 C-terminal domain complexed with the CAP-Gly domain of p150Glued
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-07-10 |
| Wavelength(s) | 0.9513 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 53.085, 80.210, 39.239 |
| Unit cell angles | 90.00, 108.52, 90.00 |
Refinement procedure
| Resolution | 17.930 - 1.800 |
| R-factor | 0.203 |
| Rwork | 0.203 |
| R-free | 0.24300 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.910 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 14411 | |
| Completeness [%] | 99.9 | 99.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | ammonium sulphate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
