1TWY
Crystal structure of an ABC-type phosphate transport receptor from Vibrio cholerae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9A |
| Synchrotron site | NSLS |
| Beamline | X9A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-05-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.009 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 84.630, 103.932, 126.687 |
| Unit cell angles | 90.00, 102.35, 90.00 |
Refinement procedure
| Resolution | 19.730 - 1.650 |
| R-factor | 0.19388 |
| Rwork | 0.193 |
| R-free | 0.23062 |
| Structure solution method | SAD |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.248 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.071 | 0.483 |
| Number of reflections | 256149 | |
| <I/σ(I)> | 22.21 | 3.02 |
| Completeness [%] | 99.7 | 99.1 |
| Redundancy | 6.82 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 6.5 | 293 | PegMME 20%, Kcl 0.2M, Tris-Bis, pH 6.5, LIQUID DIFFUSION |
| 1 | LIQUID DIFFUSION | 6.5 | 293 | PegMME 20%, Kcl 0.2M, Tris-Bis, pH 6.5, LIQUID DIFFUSION |
