1TWR
Crystal structures of ferrous and ferrous-NO forms of verdoheme in a complex with human heme oxygenase-1: catalytic implications for heme cleavage
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 119 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.038, 54.258, 70.249 |
| Unit cell angles | 90.00, 98.12, 90.00 |
Refinement procedure
| Resolution | ? - 2.100 |
| R-factor | 0.241 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qq8 |
| RMSD bond length | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.097 | 0.082 |
| Number of reflections | 59654 | |
| <I/σ(I)> | 10.3 | 2 |
| Completeness [%] | 93.1 | 94.3 |
| Redundancy | 8.1 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 8 | 301 | NH4SO4, Hepes, NaCl, pH 8.0, LIQUID DIFFUSION, temperature 301K |
