1TVX

NEUTROPHIL ACTIVATING PEPTIDE-2 VARIANT FORM M6L WITH FIVE ADDITIONAL AMINO TERMINAL RESIDUES (DSDLY)

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	273
Detector technology	AREA DETECTOR
Detector	SIEMENS
Spacegroup name	P 1 21 1
Unit cell lengths	43.810, 76.760, 43.780
Unit cell angles	90.00, 96.95, 90.00

Refinement procedure

Resolution	7.000 - 1.750
R-factor	0.196
Rwork	0.196
R-free	0.25100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	NEUTROPHIL ACTIVATING PEPTIDE-2 (1NAP)
RMSD bond length	0.014
RMSD bond angle	2.100 *
Data reduction software	XENGEN
Data scaling software	XENGEN
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	9.600
High resolution limit [Å]	1.720
Rmerge	0.039 *
Total number of observations	50642 *
Number of reflections	25385
Completeness [%]	88.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8 *	22 *	1.6M CITRATE, 0.1M HEPES, PH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium citrate	1.6 (M)
2	1	reservoir	HEPES	0.1 (M)
3	1	drop	protein	10 (mg/ml)
4	1	drop		10 (mM)