1TVR

HIV-1 RT/9-CL TIBO

Experimental procedure

Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Detector technology	CCD
Collection date	1994-11
Spacegroup name	C 1 2 1
Unit cell lengths	226.000, 69.300, 104.100
Unit cell angles	90.00, 107.00, 90.00

Refinement procedure

Resolution	10.000 - 3.000
R-factor	0.259
Rwork	0.259
RMSD bond length	0.015
RMSD bond angle	2.200
Data reduction software	DENZO
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000 *
High resolution limit [Å]	3.000 *
Rmerge	0.064
Total number of observations	56695 *
Number of reflections	26209
Completeness [%]	84.0
Redundancy	2.16

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.8 *	4 *	Clark, A.D., (1995) Methods Enzymol., 262, 171. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	19-20 (mg/ml)
2	1	drop	beta-OG	0.1 (%)
3	1	reservoir	Bis-Tris propane	50 (mM)
4	1	reservoir	ammonium sulfate	100 (mM)
5	1	reservoir	glycerol	10 (%)
6	1	reservoir	PEG8000	12 (%(w/v))
7	1	drop	DMSO	1.6 (%)