1TVL
Structure of YTNJ from Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 8-BM |
| Synchrotron site | APS |
| Beamline | 8-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9197 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 85.941, 85.941, 196.298 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.104 |
| R-factor | 0.20775 |
| Rwork | 0.207 |
| R-free | 0.22801 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.178 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.170 |
| High resolution limit [Å] | 2.104 | 2.104 |
| Rmerge | 0.095 | 0.360 |
| Number of reflections | 43146 | |
| <I/σ(I)> | 16.4 | 5.4 |
| Completeness [%] | 99.2 | 99.8 |
| Redundancy | 9.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1.5M NaCitrate, 0.1M Hepes pH 7.5, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
