1TVD

VARIABLE DOMAIN OF T CELL RECEPTOR DELTA CHAIN

Experimental procedure

Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X8C
Synchrotron site	NSLS
Beamline	X8C
Temperature [K]	278
Detector technology	CCD
Collection date	1996-06
Detector	SIEMENS
Spacegroup name	P 21 21 21
Unit cell lengths	69.940, 49.080, 61.670
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.900
R-factor	0.161
Rwork	0.161
R-free	0.22400
Structure solution method	MIRAS
RMSD bond length	0.008
RMSD bond angle	26.600 *
Data reduction software	MADNES
Data scaling software	XSCALE
Phasing software	PHASES
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.093	0.166
Total number of observations	44342 *
Number of reflections	13905 *
<I/σ(I)>	10	3
Completeness [%]	83.3	53.7
Redundancy	3	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *		Lebedeva, M.I., (1996) Protein Sci., 5, 2638. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	reservoir	sodium citrate	0.9 (M)
3	1	reservoir	sodium cacodylate	0.1 (M)	pH7.0