1TUU
Acetate Kinase crystallized with ATPgS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F2 |
Synchrotron site | CHESS |
Beamline | F2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-07-11 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0731 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 179.083, 66.111, 81.666 |
Unit cell angles | 90.00, 103.31, 90.00 |
Refinement procedure
Resolution | 29.630 - 2.500 |
R-factor | 0.213 |
Rwork | 0.213 |
R-free | 0.27600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 29.630 |
High resolution limit [Å] | 2.500 |
Number of reflections | 29904 |
Completeness [%] | 92.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 310 | Tris, ammonium sulfate, magnesium chloride, ATPgS, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 37K |