1TUU
Acetate Kinase crystallized with ATPgS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F2 |
| Synchrotron site | CHESS |
| Beamline | F2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-07-11 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0731 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 179.083, 66.111, 81.666 |
| Unit cell angles | 90.00, 103.31, 90.00 |
Refinement procedure
| Resolution | 29.630 - 2.500 |
| R-factor | 0.213 |
| Rwork | 0.213 |
| R-free | 0.27600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 29.630 |
| High resolution limit [Å] | 2.500 |
| Number of reflections | 29904 |
| Completeness [%] | 92.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 310 | Tris, ammonium sulfate, magnesium chloride, ATPgS, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 37K |
