1TUO
Crystal structure of putative phosphomannomutase from Thermus Thermophilus HB8
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL32B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-29 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.97121, 0.97920, 0.98699 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 106.610, 55.048, 83.763 |
| Unit cell angles | 90.00, 108.76, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.700 |
| Rwork | 0.221 |
| R-free | 0.24700 |
| Structure solution method | MAD |
| RMSD bond length | 1.133 |
| RMSD bond angle | 1.756 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.036 | 0.036 |
| Number of reflections | 50270 | |
| <I/σ(I)> | 19.2 | 19.2 |
| Completeness [%] | 99.0 | 99.4 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 4.4 | 291 | litium sulfate 0.5M, Na acetate 0.1M, pH 4.4, MICRO BATCH, temperature 291K |
