1TUK
Crystal structure of liganded type 2 non specific lipid transfer protein from wheat
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-09-16 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.80 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 61.465, 29.320, 41.550 |
| Unit cell angles | 90.00, 127.65, 90.00 |
Refinement procedure
| Resolution | 32.900 - 1.120 |
| R-factor | 0.138 |
| Rwork | 0.137 |
| R-free | 0.16200 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.937 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SnB |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.970 | 1.153 |
| High resolution limit [Å] | 1.120 | 1.123 |
| Number of reflections | 22535 | |
| Completeness [%] | 94.8 | 79.71 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | PEG 6000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
