1TUI

INTACT ELONGATION FACTOR TU IN COMPLEX WITH GDP

Experimental procedure

Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Detector technology	IMAGE PLATE
Collection date	1996-05-30
Detector	MARRESEARCH
Spacegroup name	P 63 2 2
Unit cell lengths	175.900, 175.900, 223.800
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.700
R-factor	0.235 *
Rwork	0.235
R-free	0.30600 *
RMSD bond length	0.020 *
RMSD bond angle	2.400 *
Data reduction software	DENZO
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *
High resolution limit [Å]	2.700 *	2.700 *
Rmerge	0.067	0.434 *
Number of reflections	56660 *	5586 *
Completeness [%]	99.9	99.9 *
Redundancy	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	EF-Tu-GDP	5 (mg/ml)
10	1	reservoir	ammonium sulfate	35 (%)
11	1	drop	protein	0.8-1.0 (mg/ml)
2	1	drop	Tris-HCl	50 (mM)
3	1	drop		10 (mM)
4	1	drop	beta-mercaptoethanol	10 (mM)
5	1	drop		0.1 (mM)
6	1	drop	GDP	10 (mM)
7	1	drop	phenyl-methyl-sulphonylfluoride	10 (mM)
8	1	drop	ammonium sulfate	25 (%)
9	1	reservoir	Tris-HCl	50 (mM)