1TUE
The X-ray Structure of the Papillomavirus Helicase in Complex with its Molecular Matchmaker E2
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2002-11-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.483,1.501 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 82.297, 88.745, 375.027 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.100 |
| R-factor | 0.22098 |
| Rwork | 0.219 |
| R-free | 0.26250 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.987 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 161300 | |
| Completeness [%] | 99.0 | 91.4 |
| Redundancy | 3.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 6 | 277 | 7-10% PEG 3,350, 50mM MES, 20-40mM MgCl, pH 6.0, Microbatch, temperature 277K |
