1TU5
Crystal structure of bovine plasma copper-containing amine oxidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-04-22 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.2 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 77.680, 131.192, 134.001 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.370 |
| R-factor | 0.205 |
| Rwork | 0.205 |
| R-free | 0.23700 |
| Structure solution method | Molecular replacement and anomalous data |
| Starting model (for MR) | 1oac 1ksi 1n9e |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.800 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.850 | 2.500 |
| High resolution limit [Å] | 2.370 | 2.370 |
| Number of reflections | 49368 | |
| <I/σ(I)> | 10.1 | 2.1 |
| Completeness [%] | 93.7 | 77.3 |
| Redundancy | 3.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.7 | 293 | 20% PEG 3350, 0.2M KH2PO4, pH 4.7, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
