1TU1
Crystal Structure of Protein of Unknown Function PA94 from Pseudomonas aeruginosa, Putative Regulator
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-24 |
| Detector | SBC-2 |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 88.247, 88.247, 107.071 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.950 |
| R-factor | 0.20527 |
| Rwork | 0.205 |
| R-free | 0.23722 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.357 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.010 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.052 | 0.478 |
| Number of reflections | 31380 | |
| <I/σ(I)> | 38.1 | 3.52 |
| Completeness [%] | 99.8 | 98.2 |
| Redundancy | 9.6 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | Ammonium sulphate, PEG 400, HEPES buffer, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
