1TTP

TRYPTOPHAN SYNTHASE (E.C.4.2.1.20) IN THE PRESENCE OF CESIUM, ROOM TEMPERATURE

Experimental procedure

Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1994-12-30
Detector	RIGAKU RAXIS IIC
Spacegroup name	C 1 2 1
Unit cell lengths	185.300, 61.300, 68.100
Unit cell angles	90.00, 94.80, 90.00

Refinement procedure

Resolution	8.000 - 2.300
R-factor	0.194 *
Rwork	0.217
R-free	0.30600 *
RMSD bond length	0.015
RMSD bond angle	3.190 *
Data reduction software	DENZO
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000
High resolution limit [Å]	2.300	2.300 *
Rmerge	0.099
Total number of observations	179673 *
Number of reflections	25385
Completeness [%]	74.2	43.2 *
Redundancy	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.8 *	20 *	pH is adjusted to 7.8 with NaOH *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	Bicine	50 (mM)
2	1	1	Na-EDTA	1 (mM)
3	1	1	spermine	0.8-1.5 (mM)
4	1	1	PEG8000	12 (%)