1TT8
CHORISMATE LYASE WITH PRODUCT, 1.0 A RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-05-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.7469 |
| Spacegroup name | P 1 |
| Unit cell lengths | 31.315, 33.849, 39.776 |
| Unit cell angles | 67.57, 87.83, 65.66 |
Refinement procedure
| Resolution | 8.000 - 1.000 |
| R-factor | 0.1231 |
| Rwork | 0.123 |
| R-free | 0.15340 |
| Structure solution method | SAD |
| Starting model (for MR) | 1fw9 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 0.035 |
| Data reduction software | X-GEN |
| Data scaling software | X-GEN |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 0.940 |
| High resolution limit [Å] | 0.920 | 0.920 |
| Rmerge | 0.066 | 0.262 |
| Number of reflections | 88226 | |
| <I/σ(I)> | 25.8 | |
| Completeness [%] | 0.9 | 0.86 |
| Redundancy | 4.6 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 297 | 26% PEG4K, 80 mM Hepes, 5%(V/V) ISOPROPANOL, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
