1TT2
Cryogenic crystal structure of Staphylococcal nuclease variant truncated Delta+PHS I92K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 95 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-01-28 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 |
| Unit cell lengths | 47.368, 47.368, 63.296 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.680 - 1.850 |
| R-factor | 0.189 |
| Rwork | 0.189 |
| R-free | 0.21100 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1snc |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | CrystalClear ((MSC/RIGAKU)) |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.680 | 1.910 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.042 | 0.113 |
| Number of reflections | 11970 | |
| <I/σ(I)> | 14.6 | 6 |
| Completeness [%] | 97.5 | |
| Redundancy | 5.84 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.1 | 277 | MPD, potassium phosphate, glycerol, DMSO, pH 8.1, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
