1TSZ

THYMIDYLATE SYNTHASE R179K MUTANT

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	295
Detector technology	AREA DETECTOR
Collection date	1989-06-29
Detector	SIEMENS
Wavelength(s)	1.5418
Spacegroup name	P 61 2 2
Unit cell lengths	78.300, 78.300, 243.200
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	7.000 - 2.750
R-factor	0.193
Rwork	0.193
RMSD bond length	0.009
RMSD bond angle	25.800 *
Data reduction software	XENGEN
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.300 *
Rmerge	0.230
Total number of observations	43932 *
Number of reflections	17573
Completeness [%]	89.0
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	2-7 (mg/ml)
2	1	drop	potassium phosphate	40 (mM)
3	1	drop	dithiothreitol	1 (mM)
4	1	drop	ammonium sulfate	0.5-2.0 (%)
5	1	reservoir	phosphate
6	1	reservoir	dithiothreitol
7	1	reservoir	EDTA