1TSY
THYMIDYLATE SYNTHASE R179K MUTANT
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 295 |
Detector technology | IMAGE PLATE |
Collection date | 1995-06-13 |
Detector | RIGAKU |
Wavelength(s) | 1.5418 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 78.800, 78.800, 242.000 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 7.000 - 2.200 |
R-factor | 0.206 |
Rwork | 0.206 |
RMSD bond length | 0.008 |
RMSD bond angle | 24.100 * |
Data reduction software | R-AXIS |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.200 |
Rmerge | 0.088 |
Total number of observations | 90968 * |
Number of reflections | 21363 |
Completeness [%] | 91.0 |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.2 | pH 7.2 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 2-7 (mg/ml) | |
2 | 1 | drop | potassium phosphate | 40 (mM) | |
3 | 1 | drop | dithiothreitol | 1 (mM) | |
4 | 1 | drop | ammonium sulfate | 0.5-2.0 (%sat) | |
5 | 1 | reservoir | phosphate | ||
6 | 1 | reservoir | dithiothreitol | ||
7 | 1 | reservoir | EDTA |