1TSY
THYMIDYLATE SYNTHASE R179K MUTANT
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-06-13 |
| Detector | RIGAKU |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 78.800, 78.800, 242.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 7.000 - 2.200 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 24.100 * |
| Data reduction software | R-AXIS |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.200 |
| Rmerge | 0.088 |
| Total number of observations | 90968 * |
| Number of reflections | 21363 |
| Completeness [%] | 91.0 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.2 | pH 7.2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 2-7 (mg/ml) | |
| 2 | 1 | drop | potassium phosphate | 40 (mM) | |
| 3 | 1 | drop | dithiothreitol | 1 (mM) | |
| 4 | 1 | drop | ammonium sulfate | 0.5-2.0 (%sat) | |
| 5 | 1 | reservoir | phosphate | ||
| 6 | 1 | reservoir | dithiothreitol | ||
| 7 | 1 | reservoir | EDTA |
