1TSR

P53 CORE DOMAIN IN COMPLEX WITH DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Temperature [K]	98
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IIC
Spacegroup name	C 1 2 1
Unit cell lengths	117.500, 67.900, 108.800
Unit cell angles	90.00, 105.50, 90.00

Refinement procedure

Resolution	6.000 - 2.200
R-factor	0.205
RMSD bond length	0.011
RMSD bond angle	2.760
Refinement software	TNT

Data quality characteristics

	Overall
Low resolution limit [Å]	100.000
High resolution limit [Å]	2.200
Rmerge	0.057
Total number of observations	123663 *
Number of reflections	36754
Completeness [%]	86.0
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.8 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	bis-tris propane-HCl	50 (mM)
2	1	drop	DNA
3	1	drop		100 (mM)
4	1	drop	dithiothreitol	10 (mM)
5	1	reservoir	PEG400	12-15 (%)
6	1	reservoir	MES	100 (mM)
7	1	reservoir	BTP-HCl	50 (mM)
8	1	reservoir	dithiothreitol	10 (mM)