1TSP
CRYSTAL STRUCTURE OF P22 TAILSPIKE PROTEIN: INTERDIGITATED SUBUNITS IN A THERMOSTABLE TRIMER
Experimental procedure
| Spacegroup name | P 21 3 |
| Unit cell lengths | 120.900, 120.900, 120.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.000 |
| R-factor | 0.184 |
| Rwork | 0.184 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.700 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 10 * | 4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 1.0 (M) | |
| 2 | 1 | reservoir | sodium phosphate | 0.1 (M) | |
| 3 | 1 | drop | protein | 10 (mg/ml) | |
| 4 | 1 | drop | HEPES | 10 (mM) | |
| 5 | 1 | drop | ammonium sulfate | 1.5 (M) | |
| 6 | 1 | drop | sodium phosphate | 0.1 (M) |
