1TSJ
Crystal structure of protein from Staphylococcus aureus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-21 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 64 |
| Unit cell lengths | 83.847, 83.847, 36.520 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.600 |
| R-factor | 0.19265 |
| Rwork | 0.189 |
| R-free | 0.26377 |
| Structure solution method | SAD |
| RMSD bond length | 0.050 |
| RMSD bond angle | 2.899 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.000 | 20.000 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.120 | |
| Number of reflections | 5836 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 32% PEG4K, 0.1M Na Acetate pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
