1TRZ

CRYSTALLOGRAPHIC EVIDENCE FOR DUAL COORDINATION AROUND ZINC IN THE T3R3 HUMAN INSULIN HEXAMER

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU R-AXIS II
Temperature [K]	290
Detector technology	IMAGE PLATE
Wavelength(s)	1.541
Spacegroup name	H 3
Unit cell lengths	80.638, 80.638, 37.782
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 1.600
R-factor	0.172
RMSD bond length	0.018
RMSD bond angle	0.049 *
Refinement software	PROFFT

Data quality characteristics

	Overall
High resolution limit [Å]	1.600 *
Rmerge	0.054
Total number of observations	34613 *
Number of reflections	11601 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6.4		Crystals were grown from 0.05M sodium citrate and 0.007M zinc acetate in the presence of 0.75M sodium chloride at pH6.4

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	sodium citrate	0.05 (M)
2	1	1	zinc acetate	0.007 (M)
3	1	2		0.75 (M)