1TRQ

X-RAY CRYSTALLOGRAPHIC AND CALORIMERIC STUDIES OF THE EFFECTS OF THE MUTATION TRP 59 TYR IN RIBONUCLEASE T1

Experimental procedure

Spacegroup name	P 1 21 1
Unit cell lengths	49.340, 48.200, 40.150
Unit cell angles	90.00, 90.29, 90.00

Refinement procedure

Resolution	10.000 - 2.300
R-factor	0.16
RMSD bond length	0.005
RMSD bond angle	1.160
Refinement software	TNT

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000 *
High resolution limit [Å]	2.300 *
Rmerge	0.050 *
Total number of observations	10020 *
Number of reflections	6966 *
Completeness [%]	74.0 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	1 (mM)
2	1	drop	2'GMP	10 (mM)
3	1	drop	sodium acetate	20 (mM)
4	1	drop	calcium acetate	2 (mM)
5	1	reservoir	MPD	51 (%(v/v))
6	1	reservoir	sodium acetate	20 (mM)