1TRN

CRYSTAL STRUCTURE OF HUMAN TRYPSIN 1: UNEXPECTED PHOSPHORYLATION OF TYROSINE 151

Experimental procedure

Source type	SYNCHROTRON
Source details	LURE BEAMLINE DW32
Synchrotron site	LURE
Beamline	DW32
Detector technology	IMAGE PLATE
Collection date	1992-10
Detector	MARRESEARCH
Spacegroup name	P 4
Unit cell lengths	107.120, 107.120, 39.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.200
R-factor	0.177
Rwork	0.177
RMSD bond length	0.010
RMSD bond angle	1.340 *
Data reduction software	MOSFLM
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000 *
High resolution limit [Å]	2.180 *	2.200 *
Rmerge	0.044
Total number of observations	63716 *
Number of reflections	22989
Completeness [%]	96.9	91.7 *
Redundancy	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5 *	20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	41 (mg/ml)
2	1	reservoir	PEG6000	14-16 (%)
3	1	reservoir	dioxane	2-3 (%(v/v))
4	1	reservoir	TAPS	50 (mM)