1TRH

TWO CONFORMATIONAL STATES OF CANDIDA RUGOSA LIPASE

Experimental procedure

Spacegroup name	C 1 2 1
Unit cell lengths	105.000, 106.700, 59.800
Unit cell angles	90.00, 94.80, 90.00

Refinement procedure

Resolution	8.000 * - 2.100
R-factor	0.148
Rwork	0.148
RMSD bond length	0.013
RMSD bond angle	2.700
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.100 *	2.100 *
Rmerge	0.064 *
Total number of observations	52852 *
Number of reflections	30450 *
Completeness [%]	81.0 *	61 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5 *		macroseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/mL)
2	1	reservoir	PEG8000	10 (%)
3	1	reservoir	cacodylate	150 (mM)