1TRE
THE STRUCTURE OF TRIOSEPHOSPHATE ISOMERASE FROM ESCHERICHIA COLI DETERMINED AT 2.6 ANGSTROM RESOLUTION
Experimental procedure
| Detector technology | IMAGE PLATE |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.690, 46.780, 151.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 * - 2.600 |
| R-factor | 0.119 |
| Rwork | 0.119 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.400 |
| Phasing software | X-PLOR |
| Refinement software | TNT |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.600 * |
| Rmerge | 0.083 * |
| Total number of observations | 51476 * |
| Number of reflections | 12801 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.6 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 10 | 1 | reservoir | dithiothreitol | 1 (mM) | |
| 11 | 1 | reservoir | EDTA | 1 (mM) | |
| 12 | 1 | reservoir | 1 (mM) | ||
| 2 | 1 | drop | Tris/Hcl | 25 (mM) | |
| 3 | 1 | drop | 20 (mM) | ||
| 4 | 1 | drop | EDTA | 1 (mM) | |
| 5 | 1 | drop | dithiothreitol | 1 (mM) | |
| 6 | 1 | drop | 1 (mM) | ||
| 7 | 1 | drop | 2-phosphoglycolate | 1 (mM) | |
| 8 | 1 | reservoir | PEG6000 | 22 (%) | |
| 9 | 1 | reservoir | MES | 200 (mM) |
