1TRB
CONVERGENT EVOLUTION OF SIMILAR FUNCTION IN TWO STRUCTURALLY DIVERGENT ENZYMES
Experimental procedure
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 123.100, 123.100, 81.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 6.000 * - 2.000 |
| R-factor | 0.177 |
| Rwork | 0.177 |
| RMSD bond length | 0.002 * |
| RMSD bond angle | 3.200 * |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.000 * |
| Rmerge | 0.005 * |
| Completeness [%] | 95.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 'Kuriyan, J.', (1989) J. Biol. Chem., 264, 12752. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG3300 | 35 (%) | |
| 2 | 1 | reservoir | ammonium sulfate | 200 (mM) | or 200 mM lithium sulfate |
| 3 | 1 | reservoir | MES | 50 (mM) | or HEPES |
| 4 | 1 | drop | protein | 20 (mg/ml) | |
| 5 | 1 | drop | dithiothreitol | 60 (mM) |
