1TR8
Crystal Structure of archaeal Nascent Polypeptide-associated Complex (aeNAC)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2003-10-14 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9795, 0.9797, 0.9117, 0.99 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 90.890, 90.890, 49.476 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.270 |
| R-factor | 0.19587 |
| Rwork | 0.194 |
| R-free | 0.24043 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.334 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | PHENIX ((HYSS)) |
| Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.390 |
| High resolution limit [Å] | 2.270 | 2.270 |
| Rmerge | 0.045 | 0.168 |
| Number of reflections | 8963 | |
| <I/σ(I)> | 8.5 | 4 |
| Completeness [%] | 96.4 | 96.4 |
| Redundancy | 6.2 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 296 | sodium malonate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
