1TR0
Crystal Structure of a boiling stable protein SP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-04-28 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.954 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 97.028, 94.750, 168.029 |
| Unit cell angles | 90.00, 90.11, 90.00 |
Refinement procedure
| Resolution | 48.500 - 1.800 |
| R-factor | 0.16447 |
| Rwork | 0.162 |
| R-free | 0.20192 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1si9 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.588 |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.500 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.447 | |
| Number of reflections | 246060 | |
| <I/σ(I)> | 2.8 | |
| Completeness [%] | 92.5 | 88.4 |
| Redundancy | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | PEG 3000, Sodium Chloride, Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
