1TQX

Crystal Structure of Pfal009167 A Putative D-Ribulose 5-Phosphate 3-Epimerase from P.falciparum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-05-11
Detector	ADSC QUANTUM 4
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	79.415, 84.454, 120.717
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	52.170 - 2.000
R-factor	0.212
Rwork	0.212
R-free	0.23300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1h1y
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	500.000
High resolution limit [Å]	2.000	2.000
Number of reflections	54930
Completeness [%]	98.7	98.7
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	298	PEG 4000, Ammonium Sulfate, Lithium Chloride, Sodium Acetate, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K