1TQW
Golgi alpha-Mannosidase II In Complex With A Diastereomer of Seleno-Salacinol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-02-03 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9504 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.007, 110.044, 138.946 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.370 - 1.200 |
| R-factor | 0.175 |
| Rwork | 0.175 |
| R-free | 0.18400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hty |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.900 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.220 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.063 | 0.453 |
| Number of reflections | 211157 | |
| <I/σ(I)> | 23.3 | 2.3 |
| Completeness [%] | 98.7 | 87.8 |
| Redundancy | 6.4 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | PEG 6000, MPD, TRIS, NaCl, pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
