1TQV

Golgi alpha-Mannosidase II In Complex With Seleno-Salacinol (Blintol)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-12-23
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	68.798, 109.527, 138.473
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.020 - 2.030
R-factor	0.143
Rwork	0.143
R-free	0.19400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hty
RMSD bond length	0.014
RMSD bond angle	1.700
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.080
High resolution limit [Å]	2.030	2.030
Rmerge	0.078	0.283
Number of reflections	68360
<I/σ(I)>	25.7	5.41
Completeness [%]	98.9	0.8
Redundancy	7.6	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7	295	PEG 6000, MPD, TRIS, NaCl, pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 295K